Accepting the award for Outstanding Performance by Male Actor in Supporting Role for his part in Quentin Tarantino's movie Once Upon a Time In Hollywood, Pitt, 56, delivered a funny line about winning the statue.
"I get to add this to my tinder profile," Angelina Jolie's ex-husband said.
Pitt then told the crowd of fellow actors that he was in awe of their performances. "You all elevated my game. I certainly hope I did the same for you.
"Let's be honest. It was a difficult part," he confessed, setting up his joke about the role he played in the movie.
"A guy who gets high, takes his shirt off and doesn't get on with his wife. It was a big stretch. Big," Pitt said as the crowd went wild.
Tom Hanks cracked up in the audience and even Aniston, 50, smiled as the camera panned to her and even Pitt laughed at his own joke.
He gushed about his love of acting and his comeback in the industry.
"Each of us in this room, we know pain, we know loneliness. We bring that to the screen...We've all had a laugh at our ridiculousness....we know funny and we bring that to the screen. And Godd**n I think that's a worthy endeavor."
Pitt and Aniston tied the knot in 2000 and split just five years later in 2005 after he met Jolie while starring in the movie Mr. and Mrs. Smith.
He and Jolie had six children together but waited until 2014 to get married. They divorced in 2017 and fought bitterly after their split.
Pitt failed to mention Maddox, Pax, Shiloh, Zahara, Knox or Vivienne during his acceptance speech again.
However, he said he had "lovely kids," during his red-carpet interview with People magazine before the show.
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Watch Brad crack a joke about his Tinder profile and his failed marriages.
Aniston smiled and clapped for Pitt after his quip about being a bad husband.
“Let’s be honest, it was a difficult part,” Pitt said. “The guy who gets high, takes his shirt off, and doesn’t get on with his wife. It’s a big stretch. Big.”
Pitt failed to mention his children with Jolie during his acceptance speech.
Aniston spoke about Lucille Ball being an influence on her career during her acceptance speech.
Pitt even kissed the statue after winning the award.
PItt said his children were "lovely," on the red carpet before the show.