Star magazine was first to report that the couple would be ending their marriage, and on Thursday Moore, 49, made it official.
"It is with great sadness and a heavy heart that I have decided to end my six-year marriage to Ashton. As a woman, a mother and a wife there are certain values and vows that I hold sacred, and it is in this spirit that I have chosen to move forward with my life. This is a trying time for me and my family, and so I would ask for the same compassion and privacy that you would give to anyone going through a similar situation," Moore said in a statement to the AP.
Moore and Kutcher, 33 were wed in September 2005.
The actress was previously married to Bruce Willis for 13 years. They have three children together, daughters Rumer, Scout and Tallulah.