On the track 'Kill Jay-Z' he says: You gave him 20 million without thinkin.
This would appear to be a direct reference to the fabled loan that he dished out to Kanye when he hit financial hard times.
The New York producer is getting very personal on his latest release and it seems that Kanye has been caught in his sights.
In the release - that broke on Thursday night - he lifts the veil on their strained relationship.
The 'Empire State of Mind' rapper also mentioned Kanye's 2004 studio album The College Dropout in the same song.
It seems the Kanye references also referred to the feud between the two, which reportedly flowered when Jay-Z failed to reach out to Kanye when his wife Kim Kardashian was robbed at gunpoint back in October.
The pair have endured a troubled relationship in recent times with Kanye also being vocal about Jay-Z. During a concert in Sacramento on November 19, Kanye stopped to rant about his relationship with the music mogul.
He told the crowd: "Jay Z — call me, 'bruh,'"
He added: "You still ain't called me. Jay Z, I know you got killers. Please don't send them at my head."
Kanye also went after Beyonce, and once again brought up the time he interrupted Taylor Swift on her behalf at the 2009 MTV Video Music Awards.
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