Joran Van Der Sloot is facing charges he murdered Stephany Flores Ramirez, and RadarOnline.com has obtained pictures of the pair that may be used in the prosecution.
Security footage at a Lima, Peru hotel show Van Der Sloot and Stephany entering his room. Additional pictures, taken hours later, show him leaving alone.
Van Der Sloot, a Dutch national, has long been the prime suspect in the 2005 disappearance of Alabama teenager Natalee Holloway.
Stephany's' badly battered body was found in Van Der Sloot's hotel room exactly five years to the day after Natalee disappeared while on vacation in Aruba. She was also last seen alive with Van Der Sloot.
Van Der Sloot was arrested for Ramirez' murder as he crossed into Chile on Thursday. He has already been extradited back to Peru to face charges.
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Natalee's family and friends are hoping the arrest and prosecution may finally lead Van Der Sloot to reveal what he knows about what happened in Aruba all those years ago.
Peru's Interior Minister Octavio Salazar said he believes Van Der Sloot may be a "serial killer."