The superstar gave birth to her twins, Sir and Rumi, on July 14 but so far she and husband Jay-Z, 47, have not invited her father Matthew to meet them.
A source told RadarOnline.com: "Matthew has not met the twins in person yet."
The proud grandpa posted a birthday message for the newborns that read: "They're here!"
While there were also stories that the singer was upset that Knowles had revealed their gender back in February.
But it is understood that he still has a strained relationship with 'Queen Bey' and that she has kept him away from the twins for now.
Knowles, 65, was at pain to set the record straight about both Beyonce and her sisterSolange in an interview with Fox 26 in Houston earlier this summer.
"I'll only say that I communicate with my daughters weekly – I would describe my relationship with my daughters as 'loving.'"
He then went on to deny that he wasn't invited to the twins birth in Los Angeles.
We pay for juicy info! Do you have a story for RadarOnline.com? Email us at email@example.com, or call us at (866) ON-RADAR (667-2327) any time, day or night.